解剖学报

›› 2012, Vol. 43 ›› Issue (3): 381-386.doi: 10.3969/j.issn.0529-1356.2012.03.017

• 肿瘤生物学 • 上一篇    下一篇

油樟叶挥发油及其主要成分的体外抗肝癌活性

叶奎川1; 殷中琼1,3* ; 魏琴 2* ; 周黎军2; 贾仁勇1,3; 徐娇1; 陶翠1   

  1. 1.四川农业大学动物医学院，四川 雅安 625014; 2.宜宾学院西南特色经济植物保护与利用重点实验室，四川 宜宾 644000; 3.四川农业大学动物疫病与人类健康四川省重点实验室，四川 雅安625014
  • 收稿日期:2011-10-31 修回日期:2011-12-08 出版日期:2012-06-06
  • 通讯作者: 殷中琼; 魏琴

Anticancer activity of the essential oil from Cinnamomum longepaniculatum leaves and its major components against human BEL-7402

  1. 1. College of Veterinary Medicine Sichuan Agricultural University, Sichuan Ya’an 625014,China; 2.Key Laboratory of Southwest Special Economic Plant Protection and Utilization, Yibin College, Sichuan, Yibin 644000, China; 3.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Sichuan Ya’an 625014, China
  • Received:2011-10-31 Revised:2011-12-08 Online:2012-06-06
  • Contact: YIN Zhong-qiong ; WEI Qin

关键词: 油樟叶挥发油, BEL-7402细胞, 四甲基偶氮唑盐法, 人

Abstract: Objective In order to study the anticancer activity of essential oil from Cinnamomum longepaniculatum leaves and its major components(1,8-cineole, sassafras oil, γ-terpinene, α-terpinene and terpinene-4-alcohol) against human BEL-7402 ceells. Methods By 3-4,5-diemethylthiarol-2,5-diphenytetrazolium bromide (MTT) fast staining method to study the growth inhibition rate of the essential oil from Cinnamomum longepaniculatum leaves and its major components on human BEL-7402 cells, and by soft agar cloning formation assay to study the effect of the essential oil from Cinnamomum longepaniculatum leaves and its major components against human BEL-7402 cells. Results The growth inhibition rates of the essential oil from Cinnamomum longepaniculatum leaves, 1,8-cineole, sassafras oil, γ-terpinene, α-terpinene and terpinene-4-alcohol against the BEL-7402 cells were 58.63%, 42.83%, 90.04%, 81.53%, 34.83% and 31.46%, respectively in vitro. Colony forming rate of the essential oil from Cinnamomum longepaniculatum leaves, 1,8-cineole, sassafras oil, γ-terpinene. α-terpinene and terpinen-4-alcohol on BEL-7402 cells were 55.13%, 61.99%, 94.56%, 89.54%, 62.02% and 59.91%, respectively. Conclusion The essential oil from Cinnamomum longepaniculatum leaves and its major components can inhibit the proliferation of live

Key words: Essential oil of Cinnamomum longepaniculatum, BEL-7402 cell, MTT, Human

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